Author/Authors :
Xu، نويسنده , , X.X. and Zhang، نويسنده , , J.X. and Guo، نويسنده , , F. and Zheng، نويسنده , , W. and Zhou، نويسنده , , H.M and Wang، نويسنده , , B.L. and Zheng، نويسنده , , Y.F. and Wang، نويسنده , , Y.B. and Cheng، نويسنده , , Y. and Lou، نويسنده , , X. and Jang، نويسنده , , B.Z.، نويسنده ,
Abstract :
In this paper, an amperometric biosensor of hydrogen peroxide (H2O2) was fabricated by immobilization of Hemoglobin (Hb) on a Pluronic P123-nanographene platelet (NGP) composite. Direct electron transfer in the Hb-immobilized P123-NGP composite film was greatly facilitated. The surface concentration (Γ*) and apparent heterogeneous electron transfer rate constant (ks) were calculated to be (1.60 ± 0.17) × 10−10 mol cm−2 and 48.51 s−1, respectively. In addition, the Hb/Pluronic P123-NGP composite showed excellent bioelectrocatalytic activity toward the reduction of H2O2. The biosensor of H2O2 exhibited a linear response to H2O2 in the range of 10–150 μM and a detection limit of 8.24 μM (S/N = 3) was obtained. The apparent Michaelis–Menten constant ( K m app ) was 45.35 μM. The resulting biosensor showed fast amperometric response, with very high sensitivity, reliability and effectiveness.
Keywords :
Nanographene platelets , Electrocatalysis , Direct electron transfer , Biosensor , Hemoglobin
