Preparative separation of conjugated linoleic acids (CLAs) from fermented Camellia oleifera Abel cake by β-cyclodextrin (β-CD) encapsulation using pH-zone-refining countercurrent chromatography

This paper concentrates on the separation of three conjugated linoleic acid (CLA) isomers (trans-9,trans-11 CLA, trans-10,cis-12 CLA and cis-9,trans-11 CLA) by β-cyclodextrin (β-CD) encapsulation using countercurrent chromatography from Camellia oleifera Abel cake fermented by lactic acid bacteria Lactobacillus sp. LL-ZSDS001. The elution sequence of the CLA isomers, the mixing zones and mechanism of separation are discussed. The separation of 305.9 mg of the crude sample yielded three isomeric compounds: 91.3 mg of trans-9,trans-11 CLA, 84.1 mg of trans-10,cis-12 CLA and 79.7 mg of cis-9,trans-11 CLA at high purities of 98%, 94% and 96%, respectively.