A novel reusable PAni-PVA-Amylase film: Activity and analysis

Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25 °C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe2+ inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity.