Author/Authors :
Abbasiliasi، نويسنده , , Sahar and Tan، نويسنده , , Joo Shun and Ibrahim، نويسنده , , Tengku Azmi Tengku and Kadkhodaei، نويسنده , , Saeid and Ng، نويسنده , , Hui Suan and Vakhshiteh، نويسنده , , Faezeh and Ajdari، نويسنده , , Zahra and Mustafa، نويسنده , , Shuhaimi and Ling، نويسنده , , Tau Chuan and Rahim، نويسنده , , Raha Abdul and Ariff، نويسنده , , Arbakariya B. Ariff ، نويسنده ,
Abstract :
A polymer–salt aqueous two-phase system (ATPS) consisting of polyethylene–glycol (PEG) with sodium citrate was developed for direct recovery of a bacteriocin-like inhibitory substance (BLIS) from a culture of Pediococcus acidilactici Kp10. The influences of phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and sodium chloride (NaCl) on the partition behaviour of BLIS was investigated. Under optimum conditions of ATPS, the purification of BLIS was achieved at 26.5% PEG (8000)/11% sodium citrate with a TLL of 46.38% (w/w), VR of 1.8, and 1.8% crude load at pH 7 without the presence of NaCl. BLIS from P. acidilactici Kp10 was successfully purified by the ATPS up to 8.43-fold with a yield of 81.18%. Given that the operation of ATPS is simple, environmentally friendly and cost-effective, as it requires only salts and PEG, it may have potential for industrial applications in the recovery of BLIS from fermentation broth.
Keywords :
Pediococcus acidilactici , Aqueous two-phase system , Bacteriocin-like inhibitory substance , Recovery
