Author/Authors :
Song، نويسنده , , F. and Zhou، نويسنده , , Y. and Li، نويسنده , , Y.S. and Meng، نويسنده , , X.M. and Meng، نويسنده , , X.Y. and Liu، نويسنده , , J.Q. and Lu، نويسنده , , S.Y. and Ren، نويسنده , , H.L. and Hu، نويسنده , , P. and Liu، نويسنده , , Z.S. and Zhang، نويسنده , , Y.Y. and Zhang، نويسنده , , J.H.، نويسنده ,
Abstract :
An immunomagnetic beads-based enzyme-linked immunosorbent assay (IMBs-ELISA) was developed for the detection of β-casein in bovine milk. Immunomagnetic beads (IMBs) were employed as the solid phase. The anti-β-casein monoclonal antibody (McAb) bound to IMBs was used as capture probe and an anti-β-casein polyclonal antibody (PcAb), labelled with horseradish peroxidase (HRP), was employed as detector probe. Three reaction and two washing steps were needed. Each reaction needed 10 min or less, which significantly shortened detection compared with classic sandwich ELISA. β-Casein in bovine milk was detected across a linear range (2–128 μg mL−1). Application results were in accordance with the Kjejdahl method, which suggests the IMBs-ELISA is rapid and reliable for the detection of β-casein in bovine milk.
Keywords :
Immunomagnetic beads , Sandwich structure , Enzyme-linked immunosorbent assay , detection , ?-casein
