Flavonol profiles in berries of wild Vitis accessions using liquid chromatography coupled to mass spectrometry and nuclear magnetic resonance spectrometry

The flavonol profiles of grape berry skins were analysed in order to assess phenotypic variation between six grapevines belonging to six different species: Vitis vinifera, Vitis candicans, Vitis champinii, Vitis amurensis, Vitis cinerea and Vitis doaniana. High-performance liquid chromatography coupled to mass spectrometry (LC–MS) and NMR spectrometry (LC-NMR) were used to separate and identify the flavonols present in these species. The combination of LC–MS and LC–NMR data resulted in the identification of eighteen flavonols. In particular, the new flavonol diglycoside and pentoside derivatives were determined. In addition, the antioxidant capacities of flavonol grape skin extracts were evaluated by using an oxygen radical absorbance capacity method (ORAC).