Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes
Aghasadeghi، Mohammad Reza نويسنده , , Delbaz، Seyed Ali نويسنده Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, IR Iran , , Sadat، Seyed Mehdi نويسنده , , Siadat، Seyed Davar نويسنده , , Shafiee Ardestani، Mehdi نويسنده Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences,Tehran, Iran , , Rahimi، Pooneh نويسنده Department of Hepatits and AIDS, Pasteur Institute of Iran, Tehran, IR Iran , , Bolhassani ، Azam نويسنده Molecular Immunology and Vaccine Research Lab, Pasteur Institute of Iran, Tehran , , Vahabpour Roudsari، Rouhollah نويسنده Department of Hepatitis and AIDS, Pasteur Institute of Iran , , Bahramali، Golnaz نويسنده Department of Hepatitis and AIDS, Pasteur Institute of Iran , , Motevalli، Fateme نويسنده Department of Hepatitis and AIDS, Pasteur Institute of Iran , , Davari، Mehdi نويسنده Department of Hepatitis and AIDS, Pasteur Institute of Iran , , Vakily، Habib نويسنده Department of Hepatitis and AIDS, Pasteur Institute of Iran , , Sharifat Salmani، Ali نويسنده Department of Biology, Science and Research Branch, Islamic Azad University , , Borhan Nobari، Maryam نويسنده Department of Hepatitis and AIDS, Pasteur Institute of Iran ,
Background: Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features. Methods: In the present study, recombinant HBsAg encapsulated into Methanobrevibacter smithii (M. smithii) archaeosomes. Balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed. Results: Frequency of IFN-γ secreting cells in the HBsAg-containing archaeosomes group was significantly higher than HBsAg and HBsAg+C/IFA groups (p≤0.05). IgG2a titer in the sera of HBsAg-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p≤0.05). Conclusion: Analysis of induced responses revealed the Immunopotentiating characteristics of M. smithii archaeosomes in the induction of T-helper 1 responses according to the dominance of IgG2a subtype and IFN-γ secreting splenocytes of immunized mice.