Author/Authors :
Zhao، Yaofeng نويسنده , , Liu، Zhancai نويسنده Department of Physics, Chemistry and Biology, Jiaozuo Teachers College, Jiaozuo, 454001 , , Yu، Shuyang نويسنده , , Wen، Sicheng نويسنده Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska University Hospital Huddinge, SE-141 86 , , Hammarstrom، Lennart نويسنده , , Rabbani، Hodjattallah نويسنده Immune and Gene Therapy Laboratory, CCK, Karolinska University Hospital Solna Department of Antigen and Antibody Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR ,
Abstract :
A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. The Xcm I digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning PCR products with 3ʹ adenosine overhang created by Taq DNA polymerase. Furthermore, two EcoR I sites were added to the construct for identification of recombinant plasmids using a single restriction enzyme. Taken together, the more efficient cloning performance and the lower cost of this vector as compared to the commercial T vector, suggests that it may be one of the best T vectors for cloning of PCR products.
