Title of article
Production of Monoclonal Antibody against Human Nestin
Author/Authors
Hadavi، Reza نويسنده Department of Antibody-Antigen Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Zarnani، Amir Hassan نويسنده Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IR Iran , , Ahmadvand، Negah نويسنده Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , , Mahmoudi، Ahmad Reza نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Bayat، Ali Ahmad نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Mahmoudian، Jafar نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Sadeghi، Mohammad Reza نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , , Soltanghoraee، Haleh نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , , Akhondi، Mohammad-Mehdi نويسنده , , Tarahomi، Majid نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Jeddi Tehrani]، Mahmood نويسنده , , Rabbani، Hodjattallah نويسنده Immune and Gene Therapy Laboratory, CCK, Karolinska University Hospital Solna Department of Antigen and Antibody Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR ,
Issue Information
فصلنامه با شماره پیاپی 5 سال 2010
Pages
9
From page
69
To page
77
Abstract
We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays.
Journal title
AJMB Avicenna Journal of Medical Biotechnology
Serial Year
2010
Journal title
AJMB Avicenna Journal of Medical Biotechnology
Record number
1982156
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