Author/Authors :
Jafari Aqdam، Meysam نويسنده Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences , , Kamali، Koorosh نويسنده Department of Public Health, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran , , Rahgozar، Mehdi نويسنده Department of Statistical, Faculty of Statistical and Computer, Welfare & Rehabilitation Sciences University, Tehran, Iran Rahgozar, Mehdi , Ohadi، Mina نويسنده , , Manoochehri، Mehdi نويسنده Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Tahami، Ali نويسنده Reproductive Biotechnology Research Centre, Avicenna Research Institute, ACECR , , Bostanshirin، Leila نويسنده Genetic Research Centre, University of Social Welfare and Rehabilitation Sciences , , KHORRAM KHORSHID، HAMID REZA نويسنده ,
Abstract :
Alzheimerʹs disease (AD) is a genetically heterogeneous neurodegenerative disease and Late-Onset type (LOAD) is the most common form of dementia affecting people over 65 years old. CALHM1 (P86L) encodes a transmembrane glycoprotein that controls cytosolic Ca2+ concentrations and Aß levels and P86L polymorphism in this gene is significantly associated with LOAD in independent case controls in a number of studies. This study was performed to determine whether this polymorphism contributes to the risk for LOAD in Iranian population. One hundred and forty one AD patients and 141 healthy controls were recruited in this study. After extraction of genomic DNA, the genotype and allele frequencies were determined in case and control subjects using PCR/RFLP method. The statistical analysis showed a significant difference in the heterozygote genotype frequency in case and control groups and polymorphic allele had a protective role between two groups. Also after stratifying the subjects by their APOE-e4 status, no significant association was observed. Our study suggests that P86L polymorphism could be a protective factor for late-onset Alzheimerʹs disease (LOAD) in Iranian population. However, to confirm these results, further study with a bigger sample size may be required.
