West Nile Virus (WNV) is an important zoo-notic agent having a wide host range. Due to its emergence with increased virulence in a wide geographical range, its monitoring becomes im-perative. Development of more rapid and sensitive molecular techniques for instance Reverse Trans-criptase-Polymerase Chain Reaction (RT-PCR), reverse transcription loop-mediated isothermal amplification (RT-LAMP) and Nucleic Acid Se-quence Based Amplification (NASBA) assays are vital for detection of the virus. Various surveil-lance techniques according to epidemiological, climatic and geographical conditions in the ex-posed area have also been developed. The surveil-lance can be set up at different levels of the WNV transmission cycle using birds, horses and mos-quitoes as sentinels.