Author/Authors :
Barkhordari، Elham نويسنده Molecular Immunology Research Center, Department of Immunology, School of Medicine, Tehran University of Medical Sciences , , Amirzargar، Ali Akbar نويسنده , , Ebrahimi Daryani، Naser نويسنده , , Mahmoudi ، Mahdi نويسنده Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. , , Ansaripour، Bita نويسنده , , Alighardashi، Maryam نويسنده Molecular Immunology Research Center, Department of Immunology, School of Medicine, Tehran University of Medical Sciences , , Ahmadi Ashtiani، Hamid Reza نويسنده Biochemistry and Nutrition Department of Zanjan Medical University, Zanjan, Iran , , Bashashati، Mohammad نويسنده Department of Gastroenterology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Iran Bashashati, Mohammad , Rezaei، Nima نويسنده ,
Abstract :
Irritable Bowel Syndrome (IBS) is a functional gastrointestinal disorder, characterized by recurrent abdominal pain and altered bowel habits. This study was performed to investigate the important role of interleukin-12 (IL-12) in intestinal inflammation. For this study seventy one patients with IBS and 140 controls were investigated. The allele and genotype frequencies of IL-12 C(-1188)A were determined using polymerase chain reaction with sequence-specific primers. The allele A was more common that the allele C in both groups of patients and controls. There was not any significant difference on IL-12 alleles and genotypes between patients and controls. The AA genotype was the most common genotypes, which was seen in 57.4% of the patients and 51.4% of the controls (p=0.53). Although frequency of the CC genotype in the control group was lower than the patient group, this difference was not significant (5.7% vs. 11.5%, respectively, p=0.16). Considering the lack of association between IL-12 C(-1188)A polymorphism and IBS, this cytokine gene polymorphism may not have significant role in the pathophysiology of disease.
