Author/Authors :
Satyavani، Kaliyamurthi نويسنده Marine Medicinal Plant Biotechnology Laboratory, Faculty of Marine Sciences, Annamalai University, Parangipettai , , Gurudeeban، Selvaraj نويسنده Marine Medicinal Plant Biotechnology Laboratory, Faculty of Marine Sciences, Annamalai University, Parangipettai , , Ramanathan، Thiruganasambandam نويسنده Marine Medicinal Plant Biotechnology Laboratory, Faculty of Marine Sciences, Annamalai University, Parangipettai , , Balasubramanian، Thangavel نويسنده Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India ,
Abstract :
Recently there has been fabulous excitement in the nano-biotechnological area for the study of nanoparticles synthesis using some natural biological system, which has led the growth advanced nanomaterials. This intention made us to assess the biologically synthesized silver nanoparticles from the leaf of Suaeda monoica (S.monoica) using 1 mM silver nitrate. The leaf extract of S.monoica incubated with 1 mM silver nitrate solution and characterized by UV- spectrometer and AFM. The effect of synthesized silver nanoparticles on Human Epidermoid Larynx Carcinoma cell line was evaluated by the MTT colorimetric technique. As a result we observed gradual change in the colour of extract from greenish to brown. The synthesized silver nanoparticles con-firmed by UV at 430 nm and spherical shape identified in the range of 31 nm under AFM. The effect of silver nanoparticles on Human Epidermoid Larynx Carcinoma cell line exhibits a dose-dependent toxicity for the cell tested and the viability of Hep-2 cells decreased to 50% (IC50) at the concentration of 500 nM. Further findings will be determined the exact mechanisms of this cost effective Nano-treatments.
