Author/Authors :
Ghorbani، Reza نويسنده , , Emamzadeh، Abdolrahman نويسنده Department of Biology, Faculty of Sciences, University of Isfahan , , Khazaie، Yahya نويسنده , , Dormiani، Kianoush نويسنده Department of Molecular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran , , Ghaedi، Kamran نويسنده , , Rabbani، Sayed Mohammad Reza نويسنده , , Foruzanfar، Mahboubeh نويسنده Department of Molecular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran , , Karbalaie، Khadijeh نويسنده Department of Cell and Molecular Biology, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran , , Karamali، Fereshteh نويسنده Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR , , Lachinani، Liana نويسنده Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology , , Kiani-Esfahani، Abbas نويسنده Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR , , Nematollahi، Marzieh نويسنده , , NASR ESFAHANI، MOHAMMAD HOSSEIN نويسنده ,
Abstract :
Background: The transcription factor Oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. Establishment of an Oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro.
Methods: In the present study, we report construction of a recombinant vector, pDB2 Oct4 promoter/EGFP, in which expression of Enhanced Green Fluorescent Protein (EGFP) was controlled by the mouse Oct-4 promoter.
Results: In transfected mouse embryonic stem cells with this vector, EGFP was predicted to be specifically expressed in pluripotency state. After transfection, high-level expression of EGFP under the control of Oct-4 promoter was observed in manipulated embryonic stem cells.
Conclusion: Thus, our new cellular reporter showed that both the properties of embryonic cells and expression the EGFP could be of great help in studying the differentiating and reprogramming mechanisms of mESCs.
