Author/Authors :
Alijani، Sadegh نويسنده Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran , , Alizadeh، Shaban Ali نويسنده Department of Surgery, School of Medicine, Arak University of Medical Sciences, Arak, Iran , , Kazemi، Ahmad نويسنده Centeral Department Of Sistan&Balochestan Telecommunication Co., Zahedan , , Kashani Khatib، Zahra نويسنده Students Scientific Research Center (SSRC), Allied Medical School, Tehran University of Medical Sciences , , Soleimani، Masoud نويسنده , , Rezvani، Mohamadreza نويسنده Department of Hematology, Allied Medical School, Tehran University of Medical Sciences , , Minayi، Neda نويسنده Department of Hematology, Faculty of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran. , , Karami، Farshid نويسنده Department of Hematology, Allied Medical School, Tehran University of Medical Sciences , , Tayebi، Behnoosh نويسنده Department of Hematology, Qaem Hospital, Mashhad University of Medical Sciences ,
Abstract :
Background: The major hemoglobin in the fetus is hemoglobin F (𝛼2𝛾2), whereas in adult humans, hemoglobin A (𝛼2𝛽2) is predominately expressed. Several studies have indicated that expression of the HbF subunit 𝛾-globin might be regulated post-transcriptionally. This could be done by small non-coding RNAs called microRNAs which target mRNAs in a sequence-specific manner and lead to translational repression or mRNA decay. The aim of this study is to evaluate the effect of miR-26b up-regulation on 𝛾-globin gene expression in K-562 cell line.
Methods: These cells were grown in RPMI 1640 and pre miR-26b and were transfected within K-562 cell line using lentiviral vector. After RNA extraction and cDNA synthesis in selected days, miRNA up-regulation was confirmed by miRNA real time PCR and then 𝛾and 𝛽chain and GATA-1 expression were investigated by RT and QRT-PCR.
Results: The viability of cells before transfection was 90%. Three and 7 days after transfection, through the use of relative Q-PCR, the 𝛾 chain expression increased 3.7, 6.8 and 3.8 folds and GATA-1 expression increased 2.1, 6.0 and 8.0 in comparison with untransfected cells.
Conclusion: The data suggest that miR-26b can be involved in the increase of 𝛾-globin gene expression in K-562 cell line. We suggest that miR-26b may be a significant therapeutic target for increasing HbF levels in patients with sickle cell disease and 𝛽-thalassemia.
