Author/Authors :
Fotouhi، Mahrokhsar نويسنده Islamic Azad University, Tonekabon Branch, Tonekabon, Iran Fotouhi, Mahrokhsar , Eslami، Gita نويسنده , , Peirovi، Habibolah نويسنده Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Peirovi, Habibolah , Hashemibahramani، Mohammad نويسنده Department of Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hashemibahramani, Mohammad , Douraghi، Masoumeh نويسنده Helicobacter pylori Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran , , Ahmadzadeh، Elahe نويسنده Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ahmadzadeh, Elahe , Jahan Fotouhi، Shams نويسنده Islamic Azad University, Tonekabon Branch, Tonekabon, Iran Jahan Fotouhi, Shams
Abstract :
Background:
Prior investigators demonstrated Helicobacter pylori as a risk factor of liver diseases. In this study
association between H. pylori and hepatocellular carcinoma was investigated.
Patients and methods:
Totally, 59 specimens of liver were collected from two health care centers affiliated to Shahid
Beheshti University of Medical Sciences, including 22 specimens of hepatocellular carcinoma, 18 specimens of cirrhosis
and 19 normal specimens of liver. Polymerase chain reaction was used to determine the presence of H. pylori in liver
samples using H. pylori gene-specific primers.
Results:
16srRNA of Helicobacter genus were found in 31.8% of hepatocellular carcinoma and 16.7% of cirrhotic
specimens, however, we could not find this gene in normal samples. Meanwhile, the presence of ureC and cagA genes
specific for H. pylori were investigated in positive specimens to confirm the H. pylori infection, however, these genes
were not detected.
Conclusion:
Helicobacter infection exists in liver of patients with hepatocellular carcinoma. However, further studies
are necessary to confirm this association
