Author/Authors :
Jahanian-Najafabadi، A. نويسنده Department of Molecular Biology, Pasteur Institute of Iran, Tehran, I.R.Iran. , , Soleimani، M. نويسنده Tasinim Biotechnology Research Center, AJA University of Medical Sciences, Tehran, I.R. Iran. , , Azadmanesh، K. نويسنده Department of Virology, Pasteur Institute of Iran, Tehran, I.R. Iran. , , Mostafavi، E. نويسنده , , Majidzadeh-A، K. نويسنده Tasinim Biotechnology Research Center, AJA University of Medical Sciences, Tehran, I.R. Iran. ,
Abstract :
Yersinia pestis which is the causative agent of pneumonic plague and distributed in all continents has led to many deaths during the history. Because of its high mortality rate, it must be diagnosed and treated at the earliest time post infection and therefore, rapid diagnostic tests are required. In the present study, we cloned the coding sequence of F1 capsular antigen of the bacteria in the pBAD/gIII plasmid for later expression and purification of the protein to produce poly and monoclonal antibodies against this antigen, and subsequently to develop rapid and efficient diagnostics tools for Y. pestis infections.
