Author/Authors :
Cai، Mingsheng نويسنده Department of Pathogenic Biology and Immunology, Guangzhou Medical University, Guangzhou, China , , Zhao، Zhiyao نويسنده Department of Pathogenic Biology and Immunology, Guangzhou Medical University, Guangzhou, China , , Zhu، Junyi نويسنده Department of Pathogenic Biology and Immunology, Guangzhou Medical University, Guangzhou, China , , Chen، Jianhong نويسنده Department of Veterinary Medicine, Foshan Science and Technology University, Foshan, China , , Wang، Bingyun نويسنده Department of Veterinary Medicine, Foshan Science and Technology University, Foshan, China , , Li، Zi نويسنده Department of Pathogenic Biology and Immunology, Guangzhou Medical University, Guangzhou, China , , Li، Meili نويسنده Department of Pathogenic Biology and Immunology, Guangzhou Medical University, Guangzhou, China ,
Abstract :
Background: Little knowledge of synonymous codon usage pattern of pseudorabies virus (PRV) genome, especially the UL31 gene in the
process for its evolution is available.
Objectives: In the present study, the codon usage bias between PRV UL31 sequence and the UL31-like sequences was identifid.
Materials and Methods: We used a comprehensive analysis on codon usage pattern in the PRV UL31 gene and the UL31-like genes of 48
reference herpesviruses by calculating codon adaptation index, ENc, RSCU and EMBOSS assays.
Results: Cluster analysis demonstrated that the codon usage bias of UL31-like genes of 49 herpesviruses had a very close relation to their
gene functions. In addition, comparison of codon preferences in the UL31 gene of PRV with those of E. coli, yeast and human showed that
there were 33 codons showing discrete usage diffrences between PRV and yeast, 24 between PRV and E. coli, but 22 between PRV and human.
Although there were slightly fewer diffrences in codon usages between PRV and human, the diffrence is unlikely to be statistically
signifiant, and experimental studies are necessary to establish the most suitable expression system for PRV UL31.
Conclusions: These results may further our comprehending of the evolution, pathogenesis and functional studies of PRV.
