Author/Authors :
-، - نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mirnejad, Reza , -، - نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, I.R. IRAN Mofazzal Jahromi, Mirza Ali , -، - نويسنده Departments of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, I.R. IRAN Al-Musawi, Sharafaldin , -، - نويسنده Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R. IRAN Pirestani, Majid , -، - نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, I.R. IRAN Fasihi Ramandi, Mahdi , -، - نويسنده Department of Immunology and Research Centre of Molecular Biology, Baqiyatallah University of Medical Sciences, Tehran, I.R. IRAN Ahmadi, Kazem , -، - نويسنده Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R. IRAN Rajayi, Hajar , -، - نويسنده Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R. IRAN Mohammad Hassan, Zuhair , -، - نويسنده Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, I.R. IRAN Kamali, Mahdi
Abstract :
Background: Curcumin as a yellow natural compound extracted from turmeric root is known it as an antibacterial agent. One of the nanoparticles ability is to decrease the defects of usual drug delivery systems. Chitosan is a low toxic, biodegradable, biocompatible and safe polymer which is used in production of nanoparticles. Nanoparticles like chitosan-tripolyphosphate (TPP) are able to increase antibacterial properties of curcumin. Materials and methods: Curcumin-loaded chitosan-TPP nanoparticles containing chitosan, curcumin and TPP salt were synthesized by ionotropic gelation methods. First, the skin of anesthetized mice was inoculated with staphylococcus aureus and pseudomonas aeruginosa suspension. Then the infected mice were treated with curcumin-loaded chitosan-TPP nanoparticles for 3 days. Following that, antibacterial characteristics of the mice treated with curcumin-loaded chitosan-TPP nanoparticles were evaluated by bacterial culture of these mice. Results: Our results showed the size of 160±10 nm and the charge of +7 ± 2 mV in curcumin-loaded chitosan-TPP nanoparticles. These nanoparticles were also spiral shape. The encapsulating efficiency of curcumin in chitosan-TPP nanoparticles was 75 ± 2%. Bacterial culture showed that curcumin-loaded chitosan-TPP nanoparticles inhibited staphylococcus aureus and pseudomonas aeruginosa growth. Conclusion: Our study demonstrated that curcumin-loaded chitosan-TPP nanoparticles can be utilized as a potent agent in treatment of Staphylococcus aureus and Pseudomonas aeruginosa infections.
