Chemical composition and acute toxicity in the water after in situ burning – A laboratory experiment

The chemical composition and toxicity of a water soluble fraction (WSF) of oil versus the underlying water after in situ burning (ISB), has been studied in a laboratory experiment. A system for allowing water sampling after ISB was developed. Seawater samples and oil were collected prior to and immediately after ISB, and chemical analysis was conducted. The chemical characterization of the water showed that the disappearance of water soluble oil components during ISB was insignificant. Acute toxicity tests with the marine copepod Calanus finmarchicus and Microtox® bioassay was performed to establish LC50/EC50 values of the water. The results were compared with regular WAF systems with unburned weathered oil, and indicated no increase in toxicity in the underlying water after ISB.