Author/Authors :
Mafra- de- Lima، Fl?via نويسنده Department of Immunology, University of S?o Paulo, ICB, Av. Professor Lineu Prestes, 1730, Butant?, S?o Paulo, SP, Brazil , , Aimbire، Fl?vio نويسنده Unifesp- ICT- Instituto de Ciências e Tecnologia, Rua Talim, 330- Jardim Aeroporto, S?o José dos Campos, SP, Brazil. , , Miranda، Humberto نويسنده Federal University Rio de Janeiro, Av. Carlos Chagas Filho, 540, Rio de Janeiro, RJ, Brazil. , , de Paula Vieira، Rodolfo نويسنده Department of Biophotonic, University Nove de Julho, Uninove, Rua Vergueiro, 235, SP, Brazil , , de Oiveira، Ana Paula Ligeiro نويسنده Department of Biophotonic, University Nove de Julho, Uninove, Rua Vergueiro, 235, SP, Brazil , , Albertini، Regiane نويسنده Department of Biophotonic, University Nove de Julho, Uninove, Rua Vergueiro, 235, SP, Brazil ,
Abstract :
Introduction: Intestinal ischemia and reperfusion (i-I/R) is an insult associated with acute respiratory distress syndrome (ARDS). Herein we evaluate the dose-response effect of low-level laser therapy (LLLT) on lung inflammation induced by i-I/R.
Methods: Mice were subjected to mesenteric artery occlusion (45 min) and killed after clamp release and intestinal reperfusion (2h). Increasing doses (1, 3, 5 and 7,5 J/cm2) of laser irradiation (660 nm) was carried out on the mice skin over the upper bronchus for 5 min after initiating reperfusion. Neutrophils activation was determined by myeloperoxidase (MPO) activity. The mRNA expression and protein concentration of inflammatory mediators IL-1B, IL-6, TNF and IL-10 in lung were measured by RT-PCR and ELISA, respectively.
Results: With exception of 1J/cm2, LLLT reduced MPO activity as well as IL-1B levels in the lungs from inflamed mice. LLLT was also markedly effective in reducing both IL-6 and TNF expression and levels in the lungs from mice submitted to i-I/R in all laser doses studied. Otherwise, LLLT significantly increased the protein levels of IL-10 in inflamed mice by i-I/R; however only in the dose of 1J/cm2.
Conclusion: We conclude that the LLLT is able to control the neutrophils activation and pro-inflammatory cytokines release into the lungs in a model of i-I/R in mice.
