Author/Authors :
Sohrabi، Nasrin نويسنده Medical Genetic Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. , , Shekari Khaniani، Mahmoud نويسنده MD, PhD, Assistant Professor, Department of Human Genetics, Faculty of Medicine, Tabriz University of Medical Sciences,Tabriz, Iran , , Mansoori Derakhshan، Sima نويسنده Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences Tabriz, Iran. ,
Abstract :
Purpose: Association between HLA-DR4–DQ8 haplotype and type 1 Diabetes Mellitus (DM-1A) was investigated in children of East Azerbaijan state of Iran because such an association has not been previously studied in this population.
Methods: HLA-typing was performed by polymerase chain reaction sequence-specific priming. For haplotype analysis, the logistic regression model was performed.
Results: Of the three investigated alleles, the frequency of DRB1*0401 was significantly higher among patients compared with that in healthy subjects (76.74% vs. 23.26%).
Conclusion: The findings of the current study are consistent with those of previous studies and show that DRB1*0401 is associated with DM-1A; the frequencies of the two other alleles were also higher among patients, although the differences were not statistically significant. Two haplotypes associated with these alleles were also surveyed, and DRB1*0401?-DQA1*0301?, and DRB1*0401?-DQA1*0301?-DQB1*0302? were the most frequent haplotypes among the patient group.
