Author/Authors :
Abbasi، Pejman نويسنده Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran. , , Kargar، Mohammad نويسنده Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, IR Iran. , , Doosti، Abbas نويسنده Department of Biology, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran Doosti, Abbas , Mardaneh، Jalal نويسنده Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran , , Ghorbani-Dalini، Sadegh نويسنده Department of Microbiology, Young Researcher’s Club, Jahrom Branch, Islamic Azad University, Jahrom, Iran Ghorbani-Dalini, Sadegh , Dehyadegari، Mohammad Ali نويسنده Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran. ,
Abstract :
Background: Enteroaggregative E. coli (EAEC) is increasingly recognized as a cause of often
persistent diarrhea in children and adults in both developing and developed countries. The aim
of the present study was to investigate the presence and the frequency of EAEC as etiologic
agent of diarrhea in Shiraz.
Methods: A total of 715 stool samples were collected from patients with diarrhea in Shiraz.
Diarrheagenic E. coli were isolated by biochemical tests and culture from 715 stool samples
collected from different hospitals. Diarrheagenic E. coli strains isolated from diarrheal stool
samples were examined for the detection of the aggR gene by Real time PCR and PCR method.
Results: In this study, a total of 101 (14.12%) diarrheagenic E. coli were isolated from 715 stool
samples collected from different hospitals. The infected patients were 58 (57%) males and 43
(43%) females. Out of these 101 diarrheagenic E. coli identified, 5 were confirmed as EAEC in
patient. The EAEC strains were isolated from 3 of the 43 females (43%) and 2 of the 58 males
( 57%) with the mean age of 11.4±1.2 age. In this study, 5 EAEC strains were isolated from one
patient with bloody diarrhea and 4 patients with watery diarrhea. The high prevalence of EAEC
isolates was also found in watery diarrhea.
Conclusion: We therefore, recommend the routine isolation and identification of EAEC strains
from patient with diarrhea in all the clinical laboratories and other pathotype diarrheagenic E.
coli in Iran.
