Composition of the organic phosphorus fraction in basidiocarps of saprotrophic and mycorrhizal fungi

In our screening, we aimed to detect phosphonates and other forms of organic phosphorus in basidiocarps and vegetative mycelia of six common basidiomycetes. Organic phosphorus-containing compounds were extracted in alkali and analysed using 31P NMR. Monoesters, diesters, pyrophosphates and polyphosphates detected in high amounts reflected the high metabolic activity in basidiocarps (growth, production of basidiospores). Phosphonates were present in all samples, in concentrations ranging from 14 mg kg−1 of the extracted phosphorus in Boletus badius basidiocarp to 140 mg kg−1 in Amanita muscaria vegetative mycelium. Detection of phosphonates in basidiocarps together with our previous evidence from laboratory experiments support the fungal production of natural phosphonates in forest ecosystems.