Author/Authors :
-، - نويسنده Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran Shirani, Kobra , -، - نويسنده Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran Behravan, Javad , -، - نويسنده Biotechnology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran Mosaffa, Fatemeh , -، - نويسنده Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mashhad University of Medical Sciences, I. R. Iran Iranshahi, Mehrdad , -، - نويسنده Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran Mehmankhah, Babak , -، - نويسنده Department of Pharmacodynamy and Toxicology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran Razavi-Azarkhiavi, Kamal , -، - نويسنده Medical Toxicology Research Center and Pharmacy School, Mashhad University of Medical Sciences, Mashhad, I. R. Iran Karimi, Gholamreza
Abstract :
Objective: Ferula szowitsiana has been widely used for medicinal purposes around the world. The anti-oxidant effect of F.szowitsiana had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from F. szowitsiana, against hydrogen peroxide (H2O2) - induced oxidative DNA damage in human lymphocytes. Materials and Methods: Human lymphocytes were incubated with H2O2 (0, 25, 50, 100, and 200 µM), galbanic acid (200 and 400 µM) and a combination of galbanic acid (200 and 400 µM) and H2O2 (25 µM) at 4 C for 30 minutes. Solvents of galbanic acid without H2O2 were used as negative controls. Results: The findings of this study demonstrated that H2O2 exposure leads to a significant concentration-dependent increase in DNA damage. Galbanic acid did not cause DNA damage compared with the control cells. Data showed that galbanic acid does not have a protective effect against H2O2-induced oxidative DNA damage in human lymphocytes. Conclusion: According to the results, it is concluded that the capability of F. szowitsiana in reducing reactive oxygen species and the anti-inflammatory property of its methanolic extract may be due to its other ingredients.
