Title of article :
Photon-counting H33D detector for biological fluorescence imaging
Author/Authors :
Michalet، نويسنده , , X. and Siegmund، نويسنده , , O.H.W. and Vallerga، نويسنده , , J.V. and Jelinsky، نويسنده , , P. and Millaud، نويسنده , , J.E. and Weiss، نويسنده , , S.، نويسنده ,
Abstract :
We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross-delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions.
Keywords :
Photon counting , Photocathode , fluorescence , Lifetime , Position sensitive anode , Quantum dot
Journal title :
Astroparticle Physics
