Title of article :
Characterizing Novel Thermophilic Amylase Producing Bacteria From Taptapani Hot Spring, Odisha, India
Author/Authors :
Kumar، Sen Sudip نويسنده Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Odisha, India , , Sangeeta، Raut نويسنده Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Odisha, India , , Soumya، Satpathy نويسنده Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur, Odisha, India , , Ranjan، Rout Prangya نويسنده Department of Biotechnology, National Institute of Technology, Warangal, Andhra Pradesh, India , , Bidyut، Bandyopadhyay نويسنده Department of Biotechnology, Oriental Institute of Science and Technology, Midnapore-721102, West Bengal, India , , Kumar، Das Mohapatra Pradeep نويسنده Department of Microbiology, Vidyasagar University, Midnapore, West Bengal, India ,
Abstract :
Background: Amylases play a vital role in biotechnological studies and rank an important position in the world enzyme market (25% to 33%). Bioprocess method of amylase production is more effective than the other sources, since the technique is easy, cost effective, fast, and the enzymes of required properties can be procured.
Objectives: The current study aimed to report the characteristics of novel amylase producing bacterial strains isolated from Taptapani hot spring, Odisha, India.
Materials and Methods: Bacterial strains were isolated by dilution plating method from the water samples collected from Taptapani Hot Spring, Odisha and screened for amylase production through starch hydrolysis. The bacterial isolates were identified morphologically, biochemically, and finally by 16S rDNA profiling.
Results: Based on the morphological, physiological, biochemical characteristics and the molecular characterization, the isolates SS1, SS2, and SS3 were identified as Bacillus barbaricus, Aeromonas veroni, and Stenotrophomonas maltophilia, respectively. The approximate molecular weight of enzymes from SS1, SS2, and SS3 strains were 19 kDa, 56 kDa and 49 kDa, respectively.
Conclusions: The current report isolates, characterizes, and demonstrates the novel heat-adapted amylase-producing bacteria SS1, SS2 and SS3 from Taptapani hot spring, indicating its potentiality and stability under acidic conditions.
