Effect of Culture Media on Improving Anther Culture Response of Rice (Oryza sativa L.)

Anther culture has been an effective breeding method to improve desirable traits, since it allows fast achievement of genetically homozygous lines. The present studies were carried out to evaluate the response of rice anthers for high frequency callus induction and green plant regeneration in different culture media and effect of in-culture mannitol treatment in callus induction media. The effect of genotype, media composition and their interaction were significant (p < 0.001) for both callus induction and green plant regeneration. Among the media, He2 medium produced highest callus induction, green plant regeneration and least albino plant development of 40.64%, 40.93% and 3.72%, respectively. Out of 13 genotypes evaluated, IR58025B with eui (25eB) was highly responsive for both callus induction as well as green plant regeneration. Mannitol with a concentration of 100 mg/L enhanced anther response by less than 1.5% compared to untreated anthers. This information can be use in development of improved parental lines through doubled haploidy.