Author/Authors :
-، - نويسنده Department of Food Technology Research, National Nutrition and Food Technology Research Institute,
Shahid Beheshti University, M.C., P.O. Box 19395-4147, Tehran, I.R. Iran Falahatpishe, Hamidreza , -، - نويسنده Department of Nutrition and
Biochemistry, School of Public Health, Tehran University of Medical Sciences, I.R. Iran Jalali, Mahmoud , -، - نويسنده Department of Nutrition and
Biochemistry, School of Public Health, Tehran University of Medical Sciences, I.R. Iran Badami, Naser , -، - نويسنده Department of Nutrition and
Biochemistry, School of Public Health, Tehran University of Medical Sciences, I.R. Iran Mardani, Nadia , -، - نويسنده Department of Food Technology Research, National Nutrition and Food Technology Research Institute,
Shahid Beheshti University, M.C., P.O. Box 19395-4147, Tehran, I.R. Iran Khosravi-Darani, Kianoush
Abstract :
This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at pHs ranging from 8 to 11 and temperatures of 20 to 50ºC. Also the impact of different carbon andnitrogen sources were investigated. The yield and fold of enzyme purification was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity)at pH>10
