Author/Authors :
J Sarsenbayeva,، Gulbanu نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk , , M Khairullin، Berik نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk , , R Sansyzbay، Abylay نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk , , M Kassenov، Markhabat نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk , , N Volgin، Yevgeniy نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk , , S Nurpeisova، Ainur نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk , , E Issagulov، Timur نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk , , V Bogdanov، Nikolai نويسنده Laboratory Control Technology and Biopreparations, Research Institute for Biological Safety Problems, Kazakhstan, Gvardeysk ,
Abstract :
In the present study with the objective of the surface protein hemagglutinin production for single radial immunodiffusion
reaction two methods of A/H5N1 recombinant strain A/AstanaRG/6:2/2009 highly purified hemagglutinin extraction
were studied. They are ion exchange chromatography and adsorption on formalinized erythrocytes using such detergents
as octyl glucoside, cetyl trimethylammonium bromide, X-100 triton. Homogeneous highly purified hemagglutinin has
been obtained using the ion exchange chromatography.
