Title of article
Intracellular Localization of FLAG-Peroxisomal Protein in Chinese Hamster Ovary (CHO) Cells
Author/Authors
-، - نويسنده Department of Biology, School of Sciences, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran Nazari Jahantigh, Malihe , -، - نويسنده Department of Biology, School of Sciences, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran Ghaedi, Kamran , -، - نويسنده Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran Nasr Isfahani, Mohamad Hossein , -، - نويسنده Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran Tanhaei, Somayeh , -، - نويسنده Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran Rabiee, Farzaneh , -، - نويسنده Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran Karbalaei, Khadijeh , -، - نويسنده Department of Basic Medical Sciences, School of Dentistry, Khorasgan Branch, Islamic Azad University, Isfahan, I.R. Iran Ostad Sharif, Maryam , -، - نويسنده Department of Stem Cell, Royan Institute, Isfahan Research Campus, P.O. Box 81589-68433, Isfahan, I.R. Iran Nematollahi, Marzieh , -، - نويسنده Cell Sciences Research Center, Royan Institute, P.O. Box 19395-4644, Tehran, I.R. Iran Baharvand, Hossein , -، - نويسنده Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, P.O. Box 81746-73471, Isfahan, I.R. Iran Razavi, Shahnaz , -، - نويسنده Department of Biology, School of Sciences, University of Isfahan, P.O. Box 81746-73441, Isfahan, I.R. Iran Miroliaei, Mehran
Issue Information
فصلنامه با شماره پیاپی 0 سال 2008
Pages
7
From page
174
To page
180
Abstract
-
Abstract
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was constructed using the method of splicing by overlap extension polymerase chain reaction (SOE PCR) and inserted into the pUcD2SRaMCSHyg eukaryotic expression vector. To investigate the intracellular localization of the PEP protein that was linked to the FLAG tandem, the constructed plasmid was used for transient transfection of the Chinese hamster Ovary (CHO) cells. The CHO cells that were transfected with the recombinant plasmid showed peroxisomal localization of FLAG-PEP as was previously shown for catalase.
Journal title
Iranian Journal of Biotechnology (IJB)
Serial Year
2008
Journal title
Iranian Journal of Biotechnology (IJB)
Record number
2037882
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