Isolation of Two Riboflavin Producer Yeasts from Environment and Optimization of Vitamin Production

Riboflavin that commonly known as vitamin B2, is an important B vitamins for maintaining human health and it has been widely used in the fields of feed and food additives and pharmaceuticals. Certain microorganisms have the potential for natural production of vitamins and some fungi, bacteria and yeasts can produce riboflavin. So the main purpose of current study was isolation the yeasts which are capable of producing riboflavin and then investigation of the effect of different sources of carbon and nitrogen on riboflavin production. Microorganisms are capable of producing vitamins, which are essential nutrients in the energy production. So in this study, different samples from soil of sugarcane and bagasse were collected and cultivated in an enrichment medium for growth of yeasts. After purification, all colonies were examined for riboflavin production. Based on spectrophotometer and chromatographic analysis, 13 isolates could produce riboflavin. Sc1 isolate from sugarcane and B5 isolate from bagasse were selected as the best riboflavin producers by 303.09?g/ml and 295.87?g/ml respectively. Then Sc1 isolate was selected to study on the effect of carbon and nitrogen sources on production. Morphological, and biochemical characteristics of the strains were examined. The polymerase chain reaction was done by using universal specific primers. According to sequencing of about 500bp of gene and comparing the sequences with existing data in GenBank, Sc1 isolate belonged to Trichosporon asahii sp. and B5 isolate belonged to Rhodosporidium diobovatom sp.