Author/Authors :
Amini ، Abdollah نويسنده Biology and anatomy dep. Proteomic lab. Medical school, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. , , Nasr-Esfahani ، Mohammad Hossin نويسنده Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IR Iran. , , Norozian، Mohsen نويسنده Biology and anatomy dep. Proteomic lab. Medical school, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. , , Reza، Mastery Farahani نويسنده Biology and anatomy dep. Proteomic lab. Medical school, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. , , Fadaei Fathabadi، Fatemeh نويسنده Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , , Heidari، Mohammad Hassan نويسنده Biology and Anatomy Department. Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Piryaei، Abbass نويسنده 1Biology and anatomy dep. Proteomic lab. Medical school, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. , , Larijani، Leila نويسنده Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Rezaei ، Tavirani نويسنده Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. , , Rajabi Bazle ، Massome نويسنده Department of Medical Biochemistry, School of Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. , , Azargashb، Eznollah نويسنده , , Bandehpor ، Moggan نويسنده Cell and molecular biology research center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran. , , Bahrami، Zohreh نويسنده Biology and Anatomy Department. Shahid Beheshti University of Medical Sciences, Tehran, Iran ,
Abstract :
Background: One of the major problems in the treatment of infertility and in vitro fertilization (IVF), as well as
for conducting tissue engineering in the case of using cell donor is reproductive failure or transplant rejection by
the recipientʹs immune system. Nuclear transfer technology may be a more accurate means for preventing from
rejection of transplanted cells. The aim of this study is to investigate the possible changes in the protein profile
of somatic cells obtained from nuclear transfer fertilization method compared with the same for normal
fertilization via studying the protein profiles of both cells and detection of the protein expression (major
histocompatibility complex (MHC)) and by means of proteomics; so that accessing new therapeutic strategies
for cell therapy and tissue engineering may be facilitated.
Methodology: This experimental study was conducted using simulated goat fibroblast cells (experimental
group) compared with goat fibroblast cells obtained from natural fertilization (control group). After culturing the
cells, the level of protein in cell suspension and MHC biomarker expression in both groups were studied and
compared using ELISA and western blotting methods.
Results: The data from ELISA technique and the gels obtained from one-dimension electrophoresis showed
decrease in the amount of protein in the experimental group (E) compared with the control group (C group).
However, such decrease was statistically insignificant (P ? 0.05). It also showed that MHC protein, which has
been considered as a biochemical marker recently, was expressed in both groups, and the cells from both control
and experimental groups were identical in terms of structure and function of the immune system.
Conclusion: Probably, the same immune response from fibroblast cells obtained from nuclear transfer
technique and fibroblast cells derived from natural fertilization indicates that fertilization method has no impact
on the production of studied fibroblast cells. Thus, it is conjectured that this can be a good prognosis for
researchers in order for using nuclear transfer method to conduct research and tissue engineering.
