Author/Authors :
Yershebulov ، ZD نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Taranov ، DS نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Abduraimov ، EO نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Barakbaev، KB نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Kondibaeva، JB نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Kaysenov، DN نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Zaynettinova ، DB نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Amanova، JT نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Sansyzbay ، AR نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 , , Sarsenbayeva، GJ نويسنده Laboratory of Microorganisms Technology Cultivation of the Research Institute for Biological Safety Problems, Republic of Kazakhstan, Zhambyl oblast, Kordai district, Guardeiskiy, 080409 ,
Abstract :
The paper presents the results of a study of cultural characteristics of the strain A/equine/Otar/764/07 (H3N8)
of equine influenza virus isolated from sick horse during the outbreak of equine influenza epizooty in the
Republic of Kazakhstan. The studies determined the optimal culture conditions for equine influenza virus strain
A/equine/Otar/764/07 (H3N8) in MDCK cell culture. The optimal infecting dose of the virus was 0,1
TCE50/cell, temperature and cultivation duration - 34 ± 0,5 ° C, 72 h, respectively, and Trypsin concentration in
medium - 2 mg / ml. Subject to the determined parameters of A/equine/Otar/764/07(H3N8) strain cultivation in
MDCK the virus-containing suspension with an infectious titer of at least 7,00 lg TCE50/ml and
hemagglutinating titer of 8,0 log2 can be obtained. The results obtained during the strain reproductive
characteristics study can be used for the development of vaccines and diagnostic kits.
