Author/Authors :
Tajik، H نويسنده Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Raeisi، M نويسنده Department of Public Health, Faculty of Health, Golestan University of Medical Sciences, Gorgan, Iran , , Razavi Rohani، S.M نويسنده Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Hashemi، M نويسنده Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , , Amin Zare، M نويسنده Post Graduated of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Naghili، H نويسنده Post Graduated of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Rozbani، D نويسنده Graduated of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Ben Ammar، D نويسنده Biochemistry-Toxicology Laboratory, University Hospital of Monastir, Tunisia ,
Abstract :
Background: Escherichia coli and Staphylococcus aureus as important food-borne patho-gens are the main concerns of food producers and consumers which create a lot of problems worldwide. The objective of this study was to investigate the inhibitory effect of monolaurin alone and in combination with EDTA on viability of E. coli and S. aureus in culture media and Iranian white cheese.
Methods: The minimum inhibitory concentration of monolaurin and EDTA was determined by broth microdilution susceptibility test. In the next stage, 108 CFU/g of E. coli and S. aureus and different concentrations of monolaurin and EDTA were added. Samples were maintained at 4 °C for 9 days. Antibacterial effect of monolaurin and EDTA was evaluated on days 0, 1, 3, 5, 7 and 9 by the specific media. Statistical analysis was made using analysis of variance (ANOVA) by SPSS program (16.0) to compare means with a significant differ-ence when p < 0.05.
Results: Monolaurin alone in both in vitro and in vivo condition had limited effect on E. coli growth but this effect was increased when it was used in combination with EDTA (p < 0.05). Monolaurin showed strong antibacterial effect on S. aureus which was increased significant-ly when used in combination with EDTA (p < 0.05). Preservation time had significant effect on antibacterial effect of monolaurin on both pathogenic bacteria (p < 0.05).
Conclusion: Monolaurin can be used in combination with EDTA to decrease contamination risks and growth of pathogenic bacteria in Iranian white cheese.
