Author/Authors :
Peyghambari، Fatemeh نويسنده Department of Anatomical Sciences, Faculty of Medical Sciences, Islamic Azad University, Yazd Branch, Yazd, Iran. , , Fayazi، Mehri نويسنده Department of Anatomical sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran , , Amanpour، Saeid نويسنده Vali-e-Asr Reproductive Health Research Centre, Tehran University of Medical Sciences, Tehran, Iran. , , Haddadi، Mahnaz نويسنده Department of Biology Faculty of Science, Arak University, Arak, Iran , , Muhammadnejad، Samad نويسنده University of Tehran , , Muhammadnejad، Ahad نويسنده Tehran University of Medical Sciences , , Abdolahpour، Samira نويسنده Tumor Model Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran , , Enkesari، Mozhgan نويسنده Tumor Model Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran. , , Mazaheri kouhanestani، Zohreh نويسنده Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran ,
Abstract :
Background: Endometrial integrin expression changes might be a reason for implantation failure in polycystic ovarian syndromes (PCOS).
Objective: Assessment of integrin genes and proteins expression upon endometrium in the PCOS experimental mouse model was the main goal of this study.
Materials and Methods: 30 NMRI female mice were equally divided into control, experimental (PCOS; received estradiol valerate (40 mg/kg)) and sham group (received; olive oil). After 8 weeks, each group was hyper stimulated by 7 IU PMSG and then, after 48hrs, 7 IU HCG was injected. Vaginal plaque was checked. After 5 days, Progesterone and estradiol levels and endometrial tissues were investigated to evaluate of ?4, ?v, B1 and B3 integrins gene and protein by qPCR method and immunohistochemistry, respectively.
Results: Tissue samples were assessed and showed that level of progesterone was significantly decreased in PCOS group. Results of molecular part in the amount of ?v, B3, B1 and ?4 gene expressions showed a great difference in B3 and ?v genes expressions between experimental groups. ?v, B3, ?4 and B1 proteins in the endometrial stroma in the control group were expressed, but they were not detected in PCOS group.
Conclusion: According to the results, integrins had different expression patterns in different areas of the endometrium; such as epithelial and stromal. It seems that in PCOS, this pattern has changed and the results might have a great influence on implantation failure. Therefore, this study suggests that a great attention to this problem may be essential in patients who are involved
