Author/Authors :
Khanaki، Korosh نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Sadeghi، Mohammad Reza نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Akhondi، Mohammad-Mehdi نويسنده , , Darabi، Masoud نويسنده Department of Biochemistry and Clinical Laboratories, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , , Mehdizadeh، Amir Hossein نويسنده Department of Management, Islamic Azad University, South Tehran Branch, Tehran, Iran , , Shabani، Mahdi نويسنده Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , , Rahimipour، Ali نويسنده Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Nouri، Mohammad نويسنده Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran ,
Abstract :
Background: Endometriosis, a common chronic inflammatory disorder, is defined
by the atypical growth of endometrium- like tissue outside of the uterus. Secretory
phospholipase A2 group IIa (sPLA2-IIa) and fatty acid binding protein4 (FABP4)
play several important roles in the inflammatory diseases.
Objective: Due to reported potential anti-inflammatory effects of ?-3 and ?-6 fatty acids, the purpose of the present study was to investigate the effects of ?-3 and ?-6 polyunsaturated fatty acids (PUFAs) on fatty acid binding protein 4 and extracellular secretory phospholipase A2IIa in cultured endometrial cells.
Materials and Methods: Ectopic and eutopic endometrial tissues obtained from 15 women were snap frozen. After thawing and tissue digestion, primary mixed stromal and endometrial epithelial cell culture was performed for 8 days in culture mediums supplemented with normal and high ratios of ?-3 and ?-6 PUFA. sPLA2-IIa in the culture medium and FABP4 level was determined using enzyme immuno assay (EIA) technique.
Results: Within ectopic endometrial cells group, the level of cellular FABP4 and extracellular sPLA2-IIa were remarkably increased under high ?-3 PUFA exposure compared with control condition (p=0.014 and p=0.04 respectively).
Conclusion: ?-3 PUFAs may increase the level of cellular FABP4 and extracellular sPLA2-IIa in ectopic endometrial cells, since sPLAIIa and FABP4 may affect endometriosis via several mechanisms, more relevant studies are encouraged to know the potential effect of increased cellular FABP4 and extracellular sPLA2-IIa on endometriosis
