Title of article
Highly Efficient Transfection of Dendritic Cells Derived from Esophageal Squamous Cell Carcinoma Patient: Optimization with Green Fluorescent Protein and Validation with Tumor RNA as a Tool for Immuno-genetherapy
Author/Authors
-، - نويسنده Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences (MUMS), P.O. Box 9196773117, Mashhad, I.R. Iran Gholamin, Mehran , -، - نويسنده Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences (MUMS), P.O. Box 9196773117, Mashhad, I.R. Iran Moaven, Omeed , -، - نويسنده Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences (MUMS), P.O. Box 9196773117, Mashhad, I.R. Iran Farshchian, Moein , -، - نويسنده Department of Surgery, Omid Hospital, MUMS, P.O. Box 13131-91389, Mashhad, Iran Rajabi-Mashhadi, Mohammad Taghi , -، - نويسنده Department of Molecular Immunology, Immunology Research Center, MUMS, P.O. Box 9196773117, Mashhad, I.R. Iran Mahmoudi, Mahmoud , -، - نويسنده Department of Immunobiochemistry, Immunology Research Center, MUMS, P.O. Box 9196773117, Mashhad, I.R. Iran Sankian, Mojtaba , -، - نويسنده Department of Medical Physics, Avicenna Research Institute, MUMS, P.O. Box 9196773117, Mashhad, I.R. Iran Sazgarnia, Ameneh , -، - نويسنده Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences (MUMS), P.O. Box 9196773117, Mashhad, I.R. Iran Ghahraman, Marta , -، - نويسنده Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences (MUMS), P.O. Box 9196773117, Mashhad, I.R. Iran Abbaszadegan, Mohammad Reza
Issue Information
فصلنامه با شماره پیاپی 0 سال 2010
Pages
6
From page
121
To page
126
Abstract
-
Abstract
This study was conducted to optimize a highly efficient mRNA transfection into dendritic cells (DC) derived from esophageal squamous cell carcinoma (ESCC) patients. Applying an electroporation technique, in vitro synthesized Green Fluorescent Protein (GFP) mRNA was transfected as an indicator into the DCs derived from a healthy donor. Flow cytometry revealed 84.9% transfection efficiency for DCs transfected with GFP mRNA. Optimized condition (500V/300 ms) yielded 79.8% efficiency in transfecting GFP mRNA into DCs from ESCC patient. Applying this efficient method, tumoral mRNA was transfected into DCs. T cells were then primed with tumor RNA/DCs and cytotoxicity assay revealed significantly higher lyses of tumor/DC vs. Mock DC; approving the optimized method for further establishment of the preclinical phase of DC-based immunotherapy for ESCC.
Journal title
Iranian Journal of Biotechnology (IJB)
Serial Year
2010
Journal title
Iranian Journal of Biotechnology (IJB)
Record number
2048745
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