Author/Authors :
Cvetkovic، Tatjana نويسنده Department of Biochemistry, School of Medicine, University of Nis, Nis, Serbia , , Stankovic، Jablan نويسنده Clinical Center Nis/Clinic of Urology, Zorana Djindjica 46, 18000 Nis, Serbia. , , Najman، Stevo نويسنده Department of Biology, School of Medicine, University of Nis, Nis, Serbia , , Pavlovic، Dusica نويسنده Department of Biochemistry, School of Medicine, University of Nis, Nis, Serbia , , Stokanovic، Dragana نويسنده Department of Pharmacology and Toxicology, School of Medicine, University of Nis, Nis, Serbia , , Vlajkovic، Slobodan نويسنده Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia , , Dakovic-Bjelakovic، Marija نويسنده Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia , , Cukuranovic، Jovana نويسنده Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia , , Zivkovic، Vladimir نويسنده Department of Anatomy, School of Medicine, University of Nis, Nis, Serbia , , Stefanovic، Vladisav نويسنده School of Medicine, University of Nis, Nis, Serbia ,
Abstract :
Background: The aim of this study was to determine oxidative stress (OS) parameters
after testicular torsion/detorsion in adult rats.
Materials and Methods: In this experimental study, male adult Wistar rats were divided
into four groups, each consisting of seven animals: group I-one hour right testicular torsion
with subsequent orchiectomy, group II-one hour right testicular torsion followed
by detorsion, group III-unilateral right-sided orchiectomy without previous torsion and
group IV-control. After 30 days, bilateral orchiectomies were performed in rats with both
testes and unilateral orchiectomies in rats with single testicles. Parameters of OS were
determined in testicular tissue and in plasma.
Results: Plasma concentrations of advanced oxidation protein products (AOPP) and
thiobarbituric acid reactive substances (TBARS) were higher (p < 0.05 and p < 0.01,
respectively), whilst the plasma concentration of the total sulfhydryl (T-SH)-groups
was lower (p < 0.05) in group I compared to the control group. Group II had higher
plasma concentrations of AOPP compared to group IV (p < 0.05), as well as significantly
increased TBARS and decreased T-SH-group levels compared to groups III
(p < 0.05 and p < 0.01, respectively) and IV (p < 0.01, for both parameters). There were
significant differences in OS markers between the ipsilateral and contralateral testis,
as well as significant correlations among levels of both plasma and tissue markers
of OS.
Conclusion: The increase in TBARS levels seen throughout the experimental period indicated
that OS development was caused by ischemia/reperfusion in the testicular tissue.
The oxidant-antioxidant system of the testicular tissue was altered during torsion as well
as detorsion.
