Author/Authors :
Zhang، Longyu نويسنده Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China , , Liu، Ying نويسنده Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China , , Xu، Yuping نويسنده Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China , , Wu، Huan نويسنده Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China , , Wei، Zhaolian نويسنده Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China , , Cao، Yunxia نويسنده Reproductive Medicine Center, First Affiliated Hospital of Anhui Medical University, Hefei, China ,
Abstract :
Background: To investigate the expression of Beclin-1 mRNA and protein in eutopic
and ectopic endometrium of women with and without endometriosis, and evaluate the
association of Beclin-1 protein expression and serum CA125 levels in the endometriosis
group due to CA125 being a well-known biomarker of endometriosis.
Materials and Methods: The expression levels (mean ± SD) of the mRNA and protein of
Beclin-1 were examined in uterine endometria from 26 women without endometriosis and in
eutopic and ectopic endometria from 26 endometriosis patients through experimental study,
as reverse transcription PCR and Western-blotting assays. Serum CA125 levels in the endometriosis
and control groups were compared and the correlation between Beclin-1 protein
expression and serum CA125 was evaluated in the endometriosis group.
Results: Both eutopic (0.12 ± 0.04, 1.25 ± 0.42) and ectopic (0.12 ± 0.05, 1.09 ± 0.50) endometriotic
tissue from 26 women with endometriosis expressed significantly lower levels of
Beclin-1 mRNA and protein than endometrium from 26 normal women (0.15 ± 0.02, 1.67 ±
0.44) (p < 0.05). Serum CA125 levels were found to be significantly higher in the endometriosis
group (p < 0.05). In addition, Beclin-1 protein expression of eutopic endometria in patients
with endometriosis was negatively correlated with serum CA125 (r= -0.57, p < 0.01).
Conclusion: The present study strongly suggests that Beclin-1 may play a role in the
formation and progression of endometriosis.
