Title of article
Immobilizing live bacteria for AFM imaging of cellular processes
Author/Authors
Kailas، نويسنده , , L. and Ratcliffe، نويسنده , , E.C. and Hayhurst، نويسنده , , E.J. and Walker، نويسنده , , M.G. and Foster، نويسنده , , S.J. and Hobbs، نويسنده , , J.K.، نويسنده ,
Pages
6
From page
775
To page
780
Abstract
Coccoid cells of the bacterial species Staphylococcus aureus have been mechanically trapped in lithographically patterned substrates and imaged under growth media using atomic force microscopy (AFM) in order to follow cellular processes. The cells are not perturbed as there is no chemical linkage to the surface. Confinement effects are minimized compared to trapping the cells in porous membranes or soft gels. S. aureus cells have been imaged undergoing cell division whilst trapped in the patterned substrates. Entrapment in lithographically patterned substrates provides a novel way for anchoring bacterial cells so that the AFM tip will not push the cells off during imaging, whilst allowing the bacteria to continue with cellular processes.
Keywords
Lithographic patterning , Cell immobilization , Staphylococcus aureus , atomic force microscopy
Journal title
Astroparticle Physics
Record number
2049569
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