Cell-mediated immune responses of lambs to experimental infection with bovine respiratory syncytial virus

Primary infection of lambs with bovine respiratory syncytial virus (RSV) was characterised by the presence of virus-specific cytotoxic cells in the peripheral blood as early as 5 days post-infection. These effector cells lysed virus-infected autologous targets in a self-restricted manner. Depletion techniques revealed that cytotoxic activity was largely due to OvCD8 + cells. Neutrophils obtained from experimentally infected lambs 5 to 10 days post-inoculation exhibited significant cytotoxic activity in the presence of bovine RSV-specific antiserum. During the same period there was a significant lymphoproliferative response to live or inactivated bovine RSV. Lymphoproliferative and cytotoxic activity coincided with the clearance of the virus from nasal secretions.