Title of article
Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples
Author/Authors
Kimberly and Akens، نويسنده , , Margarete K. and Holznagel، نويسنده , , E. and Franchini، نويسنده , , M. and Bracher، نويسنده , , Verena، نويسنده ,
Pages
7
From page
231
To page
237
Abstract
In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque® separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.
Keywords
equine , flow cytometry , Lymphocytes , immunophenotyping
Journal title
Astroparticle Physics
Record number
2053294
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