Title of article :
Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples
Author/Authors :
Kimberly and Akens، نويسنده , , Margarete K. and Holznagel، نويسنده , , E. and Franchini، نويسنده , , M. and Bracher، نويسنده , , Verena، نويسنده ,
Abstract :
In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque® separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.
Keywords :
equine , flow cytometry , Lymphocytes , immunophenotyping
Journal title :
Astroparticle Physics
