Reactivity of monoclonal antibodies of the B cell panel on PBM from BLV-infected and lymphocytotic cows

The monoclonal antibodies included in the B cell panel of the Third Workshop on Ruminant Leukocyte Antigens were tested by flow cytometry for their reactivity with peripheral blood mononuclear cells (PBM) from normal, BLV (bovine leukemia virus)-infected but non-lymphocytotic and lymphocytotic cows. Three MoAbs probably detected pan-B cell antigens. They detected an increase in the B/T cell ratio in peripheral blood from lymphocytotic cattle. However, no changes were observed in the surface phenotypes of B cells from infected animals with MoAbs of the workshop panel.