Production of monoclonal antibodies to six isotypes of horse immunoglobulin

Stable clones of 18 mouse hybridomas that produce monoclonal antibodies (MAbs) to six isotypes of horse immunoglobulin were produced. The number of hybridomas of different specificities are: four to IgGa, four to IgGb, one to IgGc, four to IgG(T), two to IgM, and three to IgA. The immunoglobulin isotypes purified from affinity columns bound with each MAb showed variable mobility in agarose–gel electrophoresis. Migration proceeded from the anode as: IgG(T), IgA, IgM, IgGc, IgGb and IgGa. The purified isotypes also demonstrated a single precipitin line when in immunoelectrophoresis against isotype-specific or polyclonal antisera, raised against the purified isotype or whole equine serum, respectively.