Author/Authors :
Kirisawa، نويسنده , , Rikio and Fukuda، نويسنده , , Takeshi and Yamanaka، نويسنده , , Hitoki and Hagiwara، نويسنده , , Katsuro and Goto، نويسنده , , Masamitsu and Obata، نويسنده , , Yuji and Yoshino، نويسنده , , Tomo-o and Iwai، نويسنده , , Hiroshi، نويسنده ,
Abstract :
cDNA generated from lipopolysaccharide-stimulated bovine peripheral blood mononuclear cells was used to amplify and clone the bovine interleukin-1 receptor antagonist (IL-1ra) using primers derived from semi-conserved regions between human and mouse IL-1ra sequences. 5′ and 3′ terminal sequences of bovine IL-1ra were amplified by 5′ and 3′ rapid amplification of cDNA ends. The deduced amino acid sequence of bovine IL-1ra demonstrated 80%, 78%, 78%, 77% and 76% homology with human, mouse, rat, rabbit and equine sequences, respectively. Recombinant bovine IL-1ra produced in Escherichia coli suppressed the growth inhibitory activity of bovine IL-1β on A375 cells in a dose-dependent manner, indicating that the present bovine IL-1ra cDNA encodes biologically active proteins.
Keywords :
Sequence , Expression , Bovine , Interleukin-1 receptor antagonist
