T-helper cells from naive to committed

T-helper cells play a central role in the onset and regulation of the antigen-specific immune response. In swine, two subpopulations of CD4 positive T-helper cells could be defined in extra-thymic compartments. Both differ in the surface antigen expression of distinct antigens. Besides the CD4+CD8− T-helper cell subpopulation representing the phenotype of T-helper cells known from other species, in swine CD4+ T-helper cells exist showing expression of CD8α and MHCII molecules. mary in vitro immune reactions after simulation with SEB the main response is distributed to the CD4+CD8− T-cell subpopulation, which contains the majority of naive T-helper cells. During the immune response CD8α as well as MHCII molecules are expressed on the surface of activated T-helper cells. This in vitro maturation is combined with a down-regulation of CD45RC. Thus, activated T-helper cells represent a cell phenotype with high similarity to the second porcine T-helper cell subpopulation. This CD4+CD8α+ subpopulation contains in its majority MHCII+ and CD45RC− cells. In a secondary in vitro immune response against classical swine fever virus only the CD4+CD8+ T-helper subpopulation is able to respond. Therefore, T-helper memory cells can be distributed to this T-lymphocyte subpopulation. In summary, naive CD4+CD8−MHCII− porcine T-helper cells show an extra-thymic maturation to committed CD4+CD8α+MHCII+ T-helper cells.