Title of article :
Production of a monoclonal antibody to canine thymus and activation-regulated chemokine (TARC) and detection of TARC in lesional skin from dogs with atopic dermatitis
Author/Authors :
Maeda، نويسنده , , Sadatoshi and Tsukui، نويسنده , , Toshihiro and Saze، نويسنده , , Koh-ichi and Masuda، نويسنده , , Kenichi and Ohno، نويسنده , , Koichi and Tsujimoto، نويسنده , , Hajime and Iwabuchi، نويسنده , , Shigehiro، نويسنده ,
Pages :
10
From page :
83
To page :
92
Abstract :
A monoclonal antibody to canine thymus and activation-regulated chemokine (TARC/CCL17) was developed to examine the association of TARC with the immunopathogenesis of canine atopic dermatitis (AD). Recombinant canine TARC was prepared using an E. coli expression system. Results of transwell chemotaxis assay demonstrated that the recombinant canine TARC showed chemotactic activity for canine lymphoid cells expressing CC chemokine receptor 4 (CCR4). Mice were then immunized with the recombinant canine TARC to obtain monoclonal antibodies. Among the monoclonal antibodies thereby obtained, one monoclonal antibody (CTA-1) was found to react with both recombinant and authentic canine TARC in ELISA and flowcytometric assays, respectively. Immunohistochemical analysis using the monoclonal antibody CTA-1 demonstrated that keratinocytes were major TARC producing cells in lesional skin of dogs with AD.
Keywords :
Dogs , atopic dermatitis , TARC/CCL-17 , Chemokine , monoclonal antibody
Journal title :
Astroparticle Physics
Record number :
2055517
Link To Document :
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