Author/Authors :
Tang، نويسنده , , W.-R. and Shioya، نويسنده , , N. and Eguchi، نويسنده , , T. and Ebata، نويسنده , , T. and Matsui، نويسنده , , J. and Takenouchi، نويسنده , , H. and Honma، نويسنده , , D. and Yasue، نويسنده , , H. and Takagaki، نويسنده , , Y. and Enosawa، نويسنده , , S. and Itagaki، نويسنده , , M. and Taguchi، نويسنده , , T. and Kiyokawa، نويسنده , , N. and Amemiya، نويسنده , , H. and Fujimoto، نويسنده , , J.، نويسنده ,
Abstract :
A battery of mouse monoclonal antibodies (mAbs) reactive with porcine peripheral blood (PB) leukocytes was generated. Among the mAbs, 6F10 was found to react probably with cluster of differentiation (CD)8 α-chain, while 7G3 and 3E12 were found to recognize γδ T-cells, as revealed by two-color flow cytometric and immunoprecipitation studies. 7G3 was shown to react with the constant (C) region of the T-cell receptor (TCR) δ-chain by the following facts: (1) 7G3 immunoprecipitated full-length TCR δ-chain protein fused with glutathione S-transferase (GST) produced by Esherichia coli and (2) 7G3 reacted with TCR δ-chain expressing Cos-7 cells transfected with either full-length or N-terminal deleted mutant cDNA, but did not react with Cos-7 cells transfected with C-terminal deleted mutant TCR δ-chain cDNA. All three mAbs produced high-quality immunostaining results on frozen sections, revealing a distinct distribution of γδ T-cells and CD8+ cells. This report precisely characterizes mAbs against porcine TCR for the first time, facilitating molecular biological investigations of the porcine immune system.
Keywords :
pig , T lymphocytes , antibodies , T-cell receptors
