Author/Authors :
Aono، نويسنده , , Shelly and Li، نويسنده , , Changqing and Zhang، نويسنده , , Guolong and Kemppainen، نويسنده , , Robert J. and Gard، نويسنده , , Julie and Lu، نويسنده , , Wuyuan and Hu، نويسنده , , Xueyou and Schwartz، نويسنده , , Dean D. and Morrison، نويسنده , , Edward E. and Dykstra، نويسنده , , Christine and Shi، نويسنده , , Jishu، نويسنده ,
Abstract :
We report the biochemical and functional properties of a novel bovine β-defensin (bBD-1). Cloned from bovine mammary papillary duct epithelia, the bBD-1 cDNA predicts a 69 amino acid propeptide that is much more similar to human β-defensin-1 (hBD-1) than to other bovine defensins. The bBD-1 gene contains two exons and one 8.5 kb intron. Using RT-PCR, we detected the bBD-1 transcript in the teat mucosa, kidney, vagina, ovary, oviduct, and colon. A synthetic bBD-1 peptide demonstrates potent antibacterial activity against Escherichia coli. The widespread expression of bBD-1 mRNA indicates that bBD-1 may play an important role in the bovine host defense against infections.
Keywords :
defensin , bBD-1 , antimicrobial peptide , hBD-1 , mBD-1 , Bovine
